The current study showed potent antibacterial efficacy of water, ethanol and methanol extracts of root, stem, leaves and flowers of Verbesina encelioides at different doses (25, 50, 75 and 100 µg/ml) against different bacterial strains, namely, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus, with the highest antibacterial activity against the selected pathogens in case of 100 µg/ml dose of different water extracts, in water extracts of stem and leaf (E. coli), Root (B. subtilis and P. aeruginosa) and fruit (S. aureus). Furthermore, the study showed antifungal efficacy of the plant extracts against different fungal strains, namely, Aspergillus flavus and Aspergillus niger, with the highest antifungal activity against A. flavus and A. niger in case of 100 µg/ml dose of different methanolic extracts. In addition to the antimicrobial efficacy, the study also highlights antioxidant and anti-diabetic activity of water, ethanol and methanol extracts of root, stem, leaves and flowers of Verbesina encelioides at different doses (25, 50, 75 and 100 µg/ml); with the highest antioxidant activity by leaf methanolic extract and flower water extract while highest anti-diabetic activity by leaf water extract and flower water extract. Cumulatively, the study provides crucial insights into antimicrobial, antioxidant and anti-diabetic activity of different parts of the plant Verbesina encelioides.