Enrichment culture Technique

Today environment change drastically due to human activity. Out of all the ecosystems terrestrial and marine ecosystems are affected majorly. Petroleum contaminated soil samples were collected Oil Garage station of Mumbai (Coastal area). Normal soil and Petroleum contaminated soil samples were physico- chemically characterized. Total Petroleum Hydrocarbons were separated by Colum Chromatography and it was analyzed by Gas Chromatography and Mass spectroscopy. Microorganisms were isolated by enrichment culture technique. 1%v/v 4T engine oil was used as a carbons source. These petroleum hydrocarbons degrading microorganisms were morphologically and biochemically also characterized.