The availability of high-quality gene expression microarray data for Arabidopsis, available in the public domain, provide a new opportunity for genome-wide exploration and discovery of genes associated with the response of a plant under abiotic stresses. Using this approach, a database of protein sequences and associated gene IDs involved in a plant’s response to salinity stress has been created around the model plant Arabidopsis thaliana. This information can be used as a resource for the identification of orthologs in any other plant with sequence information available. A list of 140 Populus genes involved in salinity stress have been identified through the bioinformatics approach. This data can be used to clone out the genes for further characterization and testing and thereafter used for targeted salinity tolerance induction programmes under genetic improvement mandate of both agricultural and forestry species. Genes involved in a plant’s response to osmotic stress, hyperosmotic salinity, cold and drought have also been identified in Arabidopsis and its counterpart ortholog has been identified in Populus. The information of tree orthologs for abiotic stress tolerance have become available for the first time in such a bigway and will lead to designing of better vectors for genetic engineering of plants in future.