Picloram

The present study reports that the increased level of boric acid in the induction medium enhanced shoot bud induction and plant regeneration in Indian Barley cultivars. Primary cultures were raised on MSB5 medium supplemented with 20.7 M picloram and subsequently subcultured and regenerated on medium with 12.47 M & 0.2 M picloram respectively. Concentration of boric acid was modified at different stages of culture. Higher level of boric acid in the induction medium not only improved shoot but induction but also plant regeneration in later stages of cultures. Regeneration frequency of 100% with 3.4 folds more number of plantlets was achieved when H3BO3 concentration in the induction was raised to 1.0 mM (10 times than in MS) and reduced to 0.1 mM (Normal MS level) in the subculture and regeneration medium. The increased level of H3BO3 in the induction medium also proved better for other recalcitrant Indian varieties.