PCR amplification

The extraction of high quality RNA is a vital technique in plant molecular biology, particularly functional genomics. The quality of RNA determines the reliability of downstream process like real time PCR, RNA Sequencing etc. In the present study, we have reported a high quality RNA extraction protocol for Prosopis juliflora and Salvadora persica. Quality and quantity of RNA was determined by using spectrophotometer and formaldehyde gel electrophoresis. The extracted RNA was thereafter used for cDNA synthesis. The primers were designed through an in silico approach using Primer-blast tool. The designed primers were successfully used to amplify the cDNA derived from mRNA from P. juliflora and S. persica. The results indicate that RNA was of good quality and fit for RT-PCR. This high throughput plant RNA extraction protocol can be used for extracting high quality RNA from these two plant species for real time PCR and other downstream applications.