Medicinal plant

Rapid shoot proliferation was established by adventitious shoot formation from inter-node segments. Shoots were directly regenerated in vitro without callusing. The concentration of plant growth regulators (PGRs) in MS medium exhibited a discrete role in the efficacy of adventitious shoot induction. Maximum number of shoot (82.02 ± 0.08) regeneration was achieved on modified MS medium supplemented with BAP 13.01 µM, Kinetin 8.87 µM and ADS 136.52 µM. Rooting of in vitro shoots occurred in three to four weeks on transfer to MS medium containing IBA (19.68 µM). About 88 % of in vitro-raised plantlets were survived under field conditions. The protocol development in present study for rapid adventitious shoot regeneration from inter-node segments of Murraya koenigii could be helpful in carrying out various genetic modifications in this economically important medicinal plant