CTAB

The present study was undertaken in curry leaf plant (Murraya koenigii), one of the most important aromatic and medical plants in the world to explore the best DNA isolation protocol. Presence of abundant quantity of secondary metabolites in curry leaf plant resulted in poor quality and quantity of isolated genomic DNA. Thus, two DNA extraction protocols namely CTAB (Cetyl Trimethyl Ammonium bromide) modified method and Dellaporta modified method were evaluated for extraction of DNA. The quantity and quality of the DNA extracted was compared using UV-spectrophotometer and agarose gel electrophoresis. CTAB modified method was found to exhibit consistently positive results in terms of both quality and quantity of DNA (A260/280) as compared to Dellaporta method. The isolated DNA was highly intact and devoid of shearing. Further, this method was explored to different species of Rutaceae family, giving significant DNA yield.