The present investigation was focused on the active compounds present in the endophytic bacterium Brevundimonas vesicularis strain JAP that has been isolated from the fresh and healthy leaves of the plant Morinda citrifolia L. Since this plant is well-known for anticancer activity, endophytic bacteria in it may have active compounds showing similar property. Extraction of the metabolites from the extracellular culture supernatant in ethyl acetate solvent was evaporated and reconstituted in ethyl alcohol and 1% propylene glycol for invitro and in vivo studies respectively. The active compounds in it were evaluated for in vivo and in vitro antitumor property. In vitro cytotoxicity study was performed by dissolving the extract in Dimethyl Sulphoxide (DMSO). The method adopted were trypan blue dye exclusion technique using DLA (Dalton Lymphoma Ascites) tumour cell of mice. In addition, the cytotoxic potential of the extract against breast cancer cell line MCF-7 was tested using MTT (3-(4,5-dimethythiazol-2yl)-2,5-diphenyl tetrazolium bromide) assay. EAC (Erhlich Ascites Carcinoma) induced in vivo antitumor study reports that there is an increase in life span of drug treated mice with higher dose followed by lower dose compared to standard cyclophosphamide. The findings suggest that the bioactive compounds in the bacterial sample labeled dose dependent reduction in tumor size, justifying the chemical constituents present in the endophytic bacteria, may in future satisfies the criteria for the drug discovery.