Antimicrobial

The current study aims to determine phytochemical profile of the plant Justicia adhatoda via usage of Gas Chromatography-Mass Spectrometry (GC-MS) analytical technique. The study shows results pertaining to GC-MS analysis of different parts of Justicia adhatoda, including, flowers, leaves, root and stem. The results of the study show presence of 24 peaks corresponding to different phytocompounds in GC-MS analysis of Justicia adhatoda flowers. Similarly, GC-MS analysis of leaves, root and stem of Justicia adhatoda revealed presence o 36, 29 and 29 peaks respectively, corresponding to different phytocompounds. Detailed literature survey showed that most of the bioactive phytocompounds present in flowers, leaves, root and stem of the plant are reported to be antioxidant, anti-inflammatory and antimicrobial in nature. Therefore, the study concludes with a message that presence of bioactive phytocompounds in different parts of Justicia adhatoda imparts its pharmacological attributes and its potential applications in amelioration of chronic oxidative and inflammatory conditions.

The main objective of this study was to assess antimicrobial and antioxidant activity of flower and leaf samples of Woodfordia fruticosa. Antimicrobial assay of the crude extracts was performed against six tested pathogenic strains including two Gram-positive bacteria viz. Staphylococcus aureus and Micromonospora sp., two Gram negative bacteria viz. Staphylococcus epidermis and Zymomonas mobilis and two fungi, Alternaria solani and Fusarium culmorum by Agar-well diffusion method. The MIC values were determined by broth dilution method. The antioxidant activity of the extracts of the flowers and leaves of Woodfordia fructicosa were investigated by using DPPH scavenging assay and reducing power of the extract. Flowers extracts of the plant has got profound and significant percent (%) DPPH scavenging activity (93.48 ± 0.26) at 200 µg/ml concentration followed by leaves (84.17 ± 0.06) at 200 µg/ml concentration. Antimicrobial screening revealed that the flower parts of the plant showed better antimicrobial activity against micro - organisms as compared to leaf part. Maximum Zone of inhibition was observed in the flower extracts against Staphylococcus aureus (22.4 ± 0.86 mm) amongst the bacteria species and against Alternaria solani (23.5 ± 0.15 mm) amongst the fungal species.