IMPACT ANALYSIS OF FLY ASH ON CULTURAL AND BIOCHEMICAL CHARACTERISTICS OF BRADYRHIZOBIUN JAPONICUM

The present investigation is an attempt to study the impact of fly ash on Rhizobium bacteria of root nodules isolated from legume plants and to study the morphological, cultural, and biochemical characteristics of bacterial strain obtained from selected legume i.e. Glycine max. Rhizobia inhabited in root nodules of plant, grown in fly ash amended soil (80% soil + 20% FA), Rhizobia was isolated and inoculated on Yeast Extract Mannitol Agar (YEMA) medium and it’s morphological, cultural and biochemical characteristics were studied. It was observed that colonies were circular or irregular; light creamish, glistering, gelatinous, convex with entire margins. The bacteria was gram negative, rod shaped, aerobic, non spore forming and slow moving bacteria arranged single, in pairs and in clusters. It showed negative chemical reaction for indole, methyl red, voges-proskauer and hydrogen sulphide, while showed positive reaction for citrate, catalase, urease and nitrate reduction. By the help of bio chemical characteristics it was confirmed that isolated bacterial culture was of Bradyrhizobium japonicum and fly ash in the concentration of 20% does not have any negative effect on the characters of Rhizobium, our findings was supported by many earlier investigations.