IAA Oxidase Activity

The intermediate products of metabolism are called metabolites. Type of metabolites that are specifically engaged in appropriate growth, development, and reproduction are called primary metabolites. These are typically carried out by organism’s physiological tasks. Primary metabolites include a wide variety of chemical molecules, including proteins, lipids, and carbohydrates; all of which are extremely important to the growth and development of plants. To assess different primary metabolites and enzymes, various plant organs such as leaves, stems, roots, and flowers of Catharanthus roseus L. have been dried and ground into powder. Carbohydrates and lipids with certain enzymes such as indole acetic acid oxidase, polyphenol oxidase, and peroxidase assay (POX) were performed. Highest concentration of lipids (0.020 ± 0.004 mg mg g-1 dw) and carbohydrates (1.546 ± 0.1666 mg mg g-1 dw) were found in the blossom of C. roseus. Leaves showed highest polyphenol oxidase and peroxidase activities with the mean values 2.729 ± 0.03 OD min-1mg -1fresh weight and 2.4640± 0.010 mM-1cm-1, respectively. Highest IAA oxidase activity was recorded in the stem, with a maximum of 0.481 ± 0.02 OD min-1mg -1fresh weight.