GCMS

Grewia asiatica L. is a medicinal plant, used for various health benefits and disease treatment. The leaves of the plant has been widely used due to its potential antimicrobial and anti-irritant properties The current research focuses on investigating the phytochemical composition of the methanolic extract of G. asiatica L. leaves using GC-MS. The methanolic extract of leaves were also analyzed for its antibacterial potential against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa and potential antifungal activity against Alternaria alternata, Aspergillus niger, Candida albicans and Fusarium solani using well diffusion assay. The chemical composition of leaf extract revealed presence of total 44 compounds namely n-Hexadecanoic acid, Octadecanoic acid, 2,3-dihydro-Benzofuran and Heneicosane. Leaf extract has shown potent antimicrobial activity against Staphylococcus aureus (IZ=16±1.88mm), Pseudomonas aeruginosa (15±2.23mm), Fusarium solani (16±0.35mm) and Aspergillus niger (13±0.34mm). The conducted research and the results observed has expanded the presence of significant antimicrobial potential in the methanolic leaf extract Grewia asiatica L., that further substantiate the use of the tested leaf extract as an effective source of natural antimicrobial compound drug and its commercial application.

The current study aims to determine phytochemical profile of the plant Justicia adhatoda via usage of Gas Chromatography-Mass Spectrometry (GC-MS) analytical technique. The study shows results pertaining to GC-MS analysis of different parts of Justicia adhatoda, including, flowers, leaves, root and stem. The results of the study show presence of 24 peaks corresponding to different phytocompounds in GC-MS analysis of Justicia adhatoda flowers. Similarly, GC-MS analysis of leaves, root and stem of Justicia adhatoda revealed presence o 36, 29 and 29 peaks respectively, corresponding to different phytocompounds. Detailed literature survey showed that most of the bioactive phytocompounds present in flowers, leaves, root and stem of the plant are reported to be antioxidant, anti-inflammatory and antimicrobial in nature. Therefore, the study concludes with a message that presence of bioactive phytocompounds in different parts of Justicia adhatoda imparts its pharmacological attributes and its potential applications in amelioration of chronic oxidative and inflammatory conditions.

Ficus racemosa L. popularly known as the cluster fig has been found effective for the treatment of various disorders like diabetes, diarrhoea, inflammatory conditions, piles, respiratory, urinary diseases, etc. The present study is therefore aimed to investigate the phytochemical constituents, GCMS analysis, antioxidant potential, total phenolic & flavonoid contents in the leaf extracts of F. racemosa. A qualitative screening of aqueous and methanolic extracts showed the presence of Alkaloids, Flavonoids, glycosides, tannins, saponin and phenolic compounds. In TLC, total 5 spots were present in the methanolic extract with different Rf values. The total flavonoid and phenolic contents are 160.4 ± 48.51 mg QE/g & 40.68 ± 9.17 mg GAE/g respectively. The dose dependent antioxidant activity was found. GS-MS analysis was done based on retention time, peak area and molecular weight. The presence of secondary metabolites depends on the solvents used and the methods applied. Many biologically active components were present as analysed by GCMS. The highest peak area of isoamyl nitrite (4.205%) was observed. The antioxidant activity could be attributed due to the presence of phenolic and flavonoids contents. Thus the present study suggests that F. racemosa leaves extract could be used as a possible therapeutic agent for a variety of diseases.