SCREENING OF SUCCESSIVE EXTRACTS OF HAMELIA PATENS FOR ANTIFUNGAL ACTIVITY

Hamelia patens plant antimicrobial activity with various successive extracts of leaf is screening. Crude extracts of methanol, Ethyl acetate, Benzene and Hexane of leaf is evaluated for antimicrobial activity by the disc diffusion method. The term of medicinal, plants comprise a variety of types of plants used in herbarium and several plants have medicinal activities. The plant was selected for screening of antimicrobial efficacy against seven selected bacterial strains viz. A.niger (MTCC- 4325), A.flavus (MTCC- 3518), A. terreus (MTCC- 4287), Penicillium chrysogenum (MTCC- 2539), Penicillium notatum (MTCC- 6477). Pet ether leaf extract (75mg/disc) posses maximum efficacy against The antifungal activity maximum against Aspergillus flavus (Inhibition zone = 13mm, Activity index=0.81) and Aspergillus terreus (Inhibition zone = 10mm, Activity index=0.58).The results of this study show maximum efficacy against various fungal strains, these compounds represent the novel leads and the future studies and analysis may allow the development of a pharmacologically acceptable pure compounds or a class of antifungal agents. Therefore, the extract can also be used for isolation of volatiles compounds with potentials so that the extract / active fraction / pure compounds can be used as antibiotics in future therapeutics.